Montmorillonite catalysis of 30-50 mer oligonucleotides: laboratory demonstration of potential steps in the origin of the RNA world.
Identifieur interne : 003322 ( Main/Exploration ); précédent : 003321; suivant : 003323Montmorillonite catalysis of 30-50 mer oligonucleotides: laboratory demonstration of potential steps in the origin of the RNA world.
Auteurs : James P. Ferris [États-Unis]Source :
- Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life [ 0169-6149 ] ; 2002.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Adenosine, Bentonite, Oligonucleotides, RNA, Uridine Monophosphate.
- chemical , metabolism : Alkaline Phosphatase, Endoribonucleases.
- Catalysis, Evolution, Chemical, Molecular Structure, Origin of Life, Time Factors.
Abstract
Elongation of the primer 32pdA(pdA)8pA proceeds by the reaction of the 5'-phosphorimidazolides of adenosine and uridine in the presence of montmorillonite clay. Daily addition of the activated nucleotides for up to 14 days results in the formation of 40-50 mers using the 5'-phosphorimidazolide of adenosine (ImpA) and 25-30 mers using the 5'-phosphorimidazolide of uridine (ImpU). The limitation on the lengths of the chains formed is not due to the inhibitors formed since the same chain lengths were formed using 2-3 times the amount of montmorillonite catalyst. The shorter oligomers formed by the addition of U monomers is not due to its greater rate of decomposition since it was found that both the A and the U adducts decompose at about the same rates. Alkaline phosphatase hydrolysis studies revealed that some of the oligomers are capped at the 5'-end to form, with ImpA, Ap32pdA(pdA)8pA(pA)n. The extent of capping depends on the reaction time and the purine or pyrimidine base in the activated mononucleotide. Hydrolysis with ribonuclease T2 followed by alkaline phosphatase determined the sites of the 3', 5'- and 2', 5'-phosphodiester bonding to the primer. The potential significance of the mineral catalyzed formation of 50 mer oligonucleotides to the origin of life based on RNA (the RNA world scenario) is discussed.
DOI: 10.1023/a:1020543312109
PubMed: 12458736
Affiliations:
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Ferris</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590, USA. ferrij@rpi.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York, 12180-3590</wicri:regionArea><wicri:noRegion>12180-3590</wicri:noRegion></affiliation></author></analytic><series><title level="j">Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life</title><idno type="ISSN">0169-6149</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine (chemistry)</term><term>Alkaline Phosphatase (metabolism)</term><term>Bentonite (chemistry)</term><term>Catalysis</term><term>Endoribonucleases (metabolism)</term><term>Evolution, Chemical</term><term>Molecular Structure</term><term>Oligonucleotides (chemistry)</term><term>Origin of Life</term><term>RNA (chemistry)</term><term>Time Factors</term><term>Uridine Monophosphate (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ()</term><term>Adénosine ()</term><term>Bentonite ()</term><term>Catalyse</term><term>Endoribonucleases (métabolisme)</term><term>Facteurs temps</term><term>Oligonucléotides ()</term><term>Origine de la vie</term><term>Phosphatase alcaline (métabolisme)</term><term>Structure moléculaire</term><term>Uridine monophosphate ()</term><term>Évolution chimique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Adenosine</term><term>Bentonite</term><term>Oligonucleotides</term><term>RNA</term><term>Uridine Monophosphate</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkaline Phosphatase</term><term>Endoribonucleases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Endoribonucleases</term><term>Phosphatase alcaline</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Catalysis</term><term>Evolution, Chemical</term><term>Molecular Structure</term><term>Origin of Life</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN</term><term>Adénosine</term><term>Bentonite</term><term>Catalyse</term><term>Facteurs temps</term><term>Oligonucléotides</term><term>Origine de la vie</term><term>Structure moléculaire</term><term>Uridine monophosphate</term><term>Évolution chimique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elongation of the primer 32pdA(pdA)8pA proceeds by the reaction of the 5'-phosphorimidazolides of adenosine and uridine in the presence of montmorillonite clay. Daily addition of the activated nucleotides for up to 14 days results in the formation of 40-50 mers using the 5'-phosphorimidazolide of adenosine (ImpA) and 25-30 mers using the 5'-phosphorimidazolide of uridine (ImpU). The limitation on the lengths of the chains formed is not due to the inhibitors formed since the same chain lengths were formed using 2-3 times the amount of montmorillonite catalyst. The shorter oligomers formed by the addition of U monomers is not due to its greater rate of decomposition since it was found that both the A and the U adducts decompose at about the same rates. Alkaline phosphatase hydrolysis studies revealed that some of the oligomers are capped at the 5'-end to form, with ImpA, Ap32pdA(pdA)8pA(pA)n. The extent of capping depends on the reaction time and the purine or pyrimidine base in the activated mononucleotide. Hydrolysis with ribonuclease T2 followed by alkaline phosphatase determined the sites of the 3', 5'- and 2', 5'-phosphodiester bonding to the primer. The potential significance of the mineral catalyzed formation of 50 mer oligonucleotides to the origin of life based on RNA (the RNA world scenario) is discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Ferris, James P" sort="Ferris, James P" uniqKey="Ferris J" first="James P" last="Ferris">James P. Ferris</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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